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KMID : 0364820120480030200
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Korean Journal of Microbiology
2012 Volume.48 No. 3 p.200 ~ p.206
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Biosynthesis of Copolyesters Consisting of 3-Hydroxyvalerate and Medium-chain-length 3-hydroxyalkanoates by the Pseudomonas aeruginosa P-5 Strain
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Woo Sang-Hee
Kim Jae-Hee
Yu Yang-Ni
Lee Young-Ha
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Abstract
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A bacterial strain capable of synthesizing polyhydroxyalkanoates (PHAs) with an unusual pattern of monomer units
was isolated from activated sludge using the enrichment culture technique. The organism, identified as Pseudomonas
aeruginosa P-5, produced polyesters consisting of 3-hydroxyvalerate and medium-chain-length (MCL) 3-hydroxyalkanoate
monomer units when C-odd alkanoic acids such as nonanoic acid and heptanoic acid were fed as the sole carbon source.
Solvent fractionation experiments using chloroform and hexane revealed that the 3-hydroxyalkanoate monomer units
in these polyesters were copolymerized. The molar concentration of 3-hydroxyvalerate in the polyesters produced
were significantly elevated up to 26 mol% by adding 1.0 g/L valeric acid as the cosubstrate. These copolyesters were
sticky with low degrees of crystallinity. The PHA synthase genes were cloned, and the deduced amino acid
sequences were determined. P. aeruginosa P-5 possessed genes encoding MCL-PHA synthases (PhaC1 and PhaC2)
but lacked the short-chain-length PHA synthase gene, suggesting that the MCL-PHA synthases from P. aeruginosa
P-5 are uniquely active for polymerizing (R)-3-hydroxyvaleryl-CoA as well as MCL (R)-3-hydroxyacyl-CoAs.
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KEYWORD
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Pseudomonas aeruginosa, 3-hydroxyvalerate, MCL-PHA synthase, polyhydroxyalkanoate
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